2 example pipetting method – Metrohm 730 Sample Changer Manuel d'utilisation

Page 118

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4.7 Pipetting with the Swing Head

730 Sample Changer, Detailed Description

115

The swing head at tower 1 moves the pipette tip to each sample
position. Using a Dosino, to which the pipetting tube is connected,
a defined sample volume is aspirated out of the vessel and pipet-
ted into a titration vessel. The titration head at tower 2 is equipped
with electrodes, burette tips, a rod or magnetic stirrer and a rinsing
device for aspirating the solution after titration and for rinsing the
electrodes. The titration is effected at this workstation. The sample
rack is also equipped with a rinsing vessel in which the pipetting
tube is rinsed before aspirating the next sample.

4.7.2 Example Pipetting Method

We describe below a method for transferring volumes in the region
of from approx. 1 to 9 mL. The method will need to be modified if
you wish to pipette significantly smaller (

µ

L) or larger volumes.

Please consult our specialists for advice.

Note that the resolution of the 700 Dosino depends on the volume
of the dispensing piston used (see page 111 and Instructions for
Use of the 700 Dosino). Therefore, choose the volume of the
dosing unit according to the volume to be pipetted.

Titraton vessel

Rinsing vessel

Titration
head at
tower 2

10 mL pipetting
tube
6.1562.100

Guiding shaft
6.1823.010

Sample vessels

759 Swing Head
at tower 1

Transfer head
6.1462.010

Sample rack
6.2041.400

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